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Image Search Results
Journal: Cytotechnology
Article Title: A high-throughput AO/PI-based cell concentration and viability detection method using the Celigo image cytometry
doi: 10.1007/s10616-016-0015-x
Figure Lengend Snippet: Comparison of Jurkat cell concentration and viability measurement for high and low concentration samples between Cellometer and Celigo image cytometers. a High and b low Jurkat cell concentration results showing comparable measurement between the two systems. It is important to note that the error bars for Celigo are much lower than those for Cellometer due to the fact that Celigo analyzes a larger number of cell samples. c Viability comparison also showed comparable results and smaller error for Celigo. The calculated p values was greater than 0.05 for all the comparison results indicating that they are statistically the same. (Color figure online)
Article Snippet: Jurkat cell preparation and AO/PI staining protocol The
Techniques: Comparison, Concentration Assay
Journal: Cytotechnology
Article Title: A high-throughput AO/PI-based cell concentration and viability detection method using the Celigo image cytometry
doi: 10.1007/s10616-016-0015-x
Figure Lengend Snippet: Bright-field and fluorescent images of AO stained Jurkat cells at different concentrations. Serial dilutions of Jurkat cells were stained with AO to generate concentration linearity plot. The images showed decreasing cell numbers as the concentration decreased. In addition, the purple outline of the Jurkat cells indicated that these cells were counted by the software. Even at very low concentration when only a few cells were present, the Celigo software was able to count each AO or PI positive cell. (Color figure online)
Article Snippet: Jurkat cell preparation and AO/PI staining protocol The
Techniques: Staining, Concentration Assay, Software
Journal: Cytotechnology
Article Title: A high-throughput AO/PI-based cell concentration and viability detection method using the Celigo image cytometry
doi: 10.1007/s10616-016-0015-x
Figure Lengend Snippet: Bright-field and combined images of AO/PI stained Jurkat cells at different viability percentages. Different viability percentages were generated by mixing live and heat-killed Jurkat cells to create a viability linearity plot. The images show decreasing AO positive cells and increasing PI positive cells as the viability decreased. In addition, the purple and blue outlines of the live and dead Jurkat cells, respectively, indicated that these cells were counted by the software. (Color figure online)
Article Snippet: Jurkat cell preparation and AO/PI staining protocol The
Techniques: Staining, Generated, Software